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Image Search Results
Journal: bioRxiv
Article Title: CBRPP: a new RNA-centric method to study RNA-protein interactions
doi: 10.1101/2020.04.09.033290
Figure Lengend Snippet: (A) Schematic representation of CBRPP approach. By fusing dCas13 and PBL enzyme together, dCas13, under the guidance of a specific crRNA, acts as an RNA tracker to bring PBL enzyme to the target RNA, then PBL enzyme biotinylates surrounding proteins of the target RNA, followed by streptavidin beads enrichment of biotinylated proteins and mass spectrometry.
Article Snippet: Antibodies used in this study include the following: anti-HA (rabbit, H6908, Sigma-Aldrich); anti-alpha-tubulin (rabbit, 11224-1-AP, Proteintech); anti-G3BP1 (mouse, sc-365338, Santa Cruz);
Techniques: Mass Spectrometry
Journal: The Journal of Experimental Medicine
Article Title: SIGN-R1 Contributes to Protection against Lethal Pneumococcal Infection in Mice
doi: 10.1084/jem.20040795
Figure Lengend Snippet: Inactivation of the mouse SIGN-R1 gene by homologous recombination. (a) Structure of the human DC-SIGN locus. (b) Structure of the mouse SIGN-R1 locus, the targeting vector, and the predicted homologous recombination event are shown. NeoR, neomycin resistance cassette; TK, thymidine kinase cassette; B, BamHI. (c) RT-PCR analysis of SIGN-R1 expression. RNA was prepared from splenocytes and PCR was performed. −/− , SIGN-R1 −/− . (d) RT-PCR analysis of SIGN-R1 expression using PCR primers throughout the cDNA to verify gene deletion. Wild-type. SIGN-R1 −/− . Wild-type. SIGN-R1 −/− . (e) Staining with ER-TR9 (anti–SIGN-R1) monoclonal antibody using horseradish peroxidase detection. (f) Co-staining with ER-TR9 monoclonal antibody (Texas red) and MOMA monoclonal antibody (FITC) (identifying marginal metallophilic macrophages). Original magnification, 10.
Article Snippet:
Techniques: Homologous Recombination, Plasmid Preparation, Reverse Transcription Polymerase Chain Reaction, Expressing, Staining